Neutrophil depletion--but not prevention of Kupffer cell activation--decreases the severity of cerulein-induced acute pancreatitis

World J Gastroenterol. 2006 Feb 28;12(8):1219-24. doi: 10.3748/wjg.v12.i8.1219.


Aim: To determine whether neutrophil depletion and Kupffer cell inhibition might combine their protective effects to decrease the severity of acute pancreatitis.

Methods: Mice had cerulein administration to induce acute pancreatitis and were pretreated with either anti-mouse neutrophil serum or gadolinium chloride (GdCl3) to prevent Kupffer cell activation, or both treatments. Injury was assessed in pancreas and lungs. Myeloperoxidases (MPO) assessed neutrophil infiltration. Interleukin-6 (IL-6) and IL-10 were measured in serum, pancreas, lungs and liver.

Results: In mice with acute pancreatitis, neutrophil depletion reduced the severity of pancreatitis and pancreatitis-associated lung injury. Kupffer cell inactivation by GdCl3 had less protective effect, although IL-6 and IL-10 concentrations were significantly decreased. The protective treatment brought by neutrophil depletion was not enhanced by Kupffer cell inactivation and both treatments did not combine their protective effects.

Conclusion: Our results confirm the role of activated neutrophils in aggravating organ injury in acute pancreatitis while the role of Kupffer cell activation is less obvious.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acute Disease
  • Amylases / blood
  • Animals
  • Cell Count
  • Ceruletide
  • Gadolinium / pharmacology
  • Interleukins / analysis
  • Interleukins / blood
  • Kupffer Cells / drug effects
  • Kupffer Cells / physiology*
  • Liver / chemistry
  • Liver / pathology
  • Lung / chemistry
  • Lung / pathology
  • Lung / physiopathology
  • Macrophage Activation*
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Neutrophil Activation
  • Neutrophils / immunology*
  • Pancreas / chemistry
  • Pancreas / pathology
  • Pancreatitis / chemically induced
  • Pancreatitis / immunology*
  • Pancreatitis / pathology*
  • Peroxidase / analysis
  • Random Allocation
  • Severity of Illness Index


  • Interleukins
  • Ceruletide
  • Gadolinium
  • Peroxidase
  • Amylases
  • gadolinium chloride