Value of mink vomit model in study of anti-emetic drugs

World J Gastroenterol. 2006 Feb 28;12(8):1300-2. doi: 10.3748/wjg.v12.i8.1300.

Abstract

Aim: To establish a new, reliable vomit model of minks.

Methods: Adult male minks were randomly divided into 8 groups (n=6): cisplatin (7.5 mg/kg) intraperitoneal injection (i.p.) group, copper sulfate (40 mg/kg) intragastric injection (i.g.) group, apomorphine (1.6 mg/kg) subcutaneous injection (s.c.) group, and 18 Gy whole-body X-irradiation group, ondansetron injection group (2 mg/kg i.p.) 30 min later followed by cisplatin (7.5 mg/kg) i.p., normal saline (NS) i.p. injection control group, metoclopramide injection group (4 mg/kg i.p.) 30 min later followed by apomorphine (1.6 mg/kg) s.c., NS i.g. control group. The frequency of retching and vomiting was calculated. After behavioral experiment, distribution of 5-HT in the ileum was detected by immunohistologic method.

Results: Cisplatin, apomorphine, copper sulfate and X-irradiation administered to minks evoked a profound emetic response in the animals. However, retching and vomiting were significantly inhibited by pretreatment with ondansetron and metoclopramide in cisplatin and copper sulfate groups (P=0.018). Immunohistologic result showed that 5-HT released from enterochromaffin cells (EC cells) was involved in vomiting mechanism.

Conclusion: Mink vomit model has a great value in studying the vomiting mechanism and screening new antiemetic drugs.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antiemetics / therapeutic use*
  • Apomorphine
  • Cisplatin / administration & dosage
  • Copper Sulfate / administration & dosage
  • Disease Models, Animal*
  • Enterochromaffin Cells / metabolism
  • Immunohistochemistry
  • Male
  • Metoclopramide / administration & dosage
  • Metoclopramide / therapeutic use
  • Mink
  • Ondansetron / administration & dosage
  • Ondansetron / therapeutic use
  • Random Allocation
  • Reproducibility of Results
  • Serotonin / metabolism
  • Vomiting / chemically induced
  • Vomiting / physiopathology
  • Vomiting / prevention & control*

Substances

  • Antiemetics
  • Serotonin
  • Ondansetron
  • Metoclopramide
  • Copper Sulfate
  • Apomorphine
  • Cisplatin