Abstract
The VP6 gene of human group C rotavirus was cloned and sequenced. Hybridization to the human group C and the porcine group C/Cowden dsRNA genomes assigned this coding sequence to segment 5. The complete human VP6 sequence contained an open reading frame of 1185 nucleotides (395 amino acids; deduced Mr 44,669 Da). The protein sequence demonstrated low homology with the group A VP6 sequences (41.7 to 42.7%) and high homology (88.9%) with the porcine group C VP6 sequence. However, the protein sequence alignments revealed a region of 10 amino acids that were significantly different between the human and the porcine group C viruses.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Antigens, Viral*
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Base Sequence
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Blotting, Northern
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Capsid / genetics*
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Capsid Proteins*
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Cloning, Molecular
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DNA / genetics
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Genes, Viral
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Humans
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Molecular Sequence Data
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Oligonucleotides / chemistry
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Polymerase Chain Reaction
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RNA, Viral / genetics
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Rotavirus / genetics*
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Viral Structural Proteins / genetics
Substances
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Antigens, Viral
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Capsid Proteins
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Oligonucleotides
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RNA, Viral
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VP6 protein, Rotavirus
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Viral Structural Proteins
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DNA
Associated data
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GENBANK/M72882
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GENBANK/M72883
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GENBANK/M73051
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GENBANK/M73052
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GENBANK/M73053
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GENBANK/M73054
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GENBANK/M73055
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GENBANK/M73056
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GENBANK/S56131
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GENBANK/S56173
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GENBANK/X59843