Molecular cloning, sequence analysis and coding assignment of the major inner capsid protein gene of human group C rotavirus

Virology. 1991 Oct;184(2):781-5. doi: 10.1016/0042-6822(91)90452-h.

Abstract

The VP6 gene of human group C rotavirus was cloned and sequenced. Hybridization to the human group C and the porcine group C/Cowden dsRNA genomes assigned this coding sequence to segment 5. The complete human VP6 sequence contained an open reading frame of 1185 nucleotides (395 amino acids; deduced Mr 44,669 Da). The protein sequence demonstrated low homology with the group A VP6 sequences (41.7 to 42.7%) and high homology (88.9%) with the porcine group C VP6 sequence. However, the protein sequence alignments revealed a region of 10 amino acids that were significantly different between the human and the porcine group C viruses.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Antigens, Viral*
  • Base Sequence
  • Blotting, Northern
  • Capsid / genetics*
  • Capsid Proteins*
  • Cloning, Molecular
  • DNA / genetics
  • Genes, Viral
  • Humans
  • Molecular Sequence Data
  • Oligonucleotides / chemistry
  • Polymerase Chain Reaction
  • RNA, Viral / genetics
  • Rotavirus / genetics*
  • Viral Structural Proteins / genetics

Substances

  • Antigens, Viral
  • Capsid Proteins
  • Oligonucleotides
  • RNA, Viral
  • VP6 protein, Rotavirus
  • Viral Structural Proteins
  • DNA

Associated data

  • GENBANK/M72882
  • GENBANK/M72883
  • GENBANK/M73051
  • GENBANK/M73052
  • GENBANK/M73053
  • GENBANK/M73054
  • GENBANK/M73055
  • GENBANK/M73056
  • GENBANK/S56131
  • GENBANK/S56173
  • GENBANK/X59843