Skip to main page content
Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
, 61 (10), 3515-20

Lignin Peroxidases, Manganese Peroxidases, and Other Ligninolytic Enzymes Produced by Phlebia Radiata During Solid-State Fermentation of Wheat Straw

Lignin Peroxidases, Manganese Peroxidases, and Other Ligninolytic Enzymes Produced by Phlebia Radiata During Solid-State Fermentation of Wheat Straw

T Vares et al. Appl Environ Microbiol.

Abstract

The white rot fungus Phlebia radiata 79 (ATCC 64658) produces lignin peroxidase (LiP), manganese peroxidase (MnP), glyoxal oxidase (GLOX), and laccase in the commonly used glucose low-nitrogen liquid medium. However, the enzymes which this fungus utilizes for selective removal of lignin during degradation of different lignocellulosic substrates have not been studied before. Multiple forms of LiP, MnP, GLOX, and laccase were purified from P. radiata culture extracts obtained after solid-state fermentation of wheat straw. However, the patterns of extracellular lignin-modifying enzymes studied were different from those of the enzymes usually found in liquid cultures of P. radiata. Three LiP isoforms were purified. The major LiP isoform from solid-state cultivation was LiP2. LiP3, which has usually been described as the major isoenzyme in liquid cultures, was not expressed during straw fermentation. New MnP isoforms have been detected in addition to the previously reported MnPs. GLOX was secreted in rather high amounts simultaneously with LiP during the first 2 weeks of growth. GLOX purified from P. radiata showed multiple forms, with pIs ranging from 4.0 to 4.6 and with a molecular mass of ca. 68 kDa.

Similar articles

See all similar articles

Cited by 17 PubMed Central articles

See all "Cited by" articles

References

    1. Anal Biochem. 1976 May 7;72:248-54 - PubMed
    1. Biochemistry. 1994 Nov 15;33(45):13349-54 - PubMed
    1. Proc Natl Acad Sci U S A. 1994 Dec 20;91(26):12794-7 - PubMed
    1. Appl Environ Microbiol. 1993 Jan;59(1):260-5 - PubMed
    1. Microbiology. 1994 Oct;140 ( Pt 10):2691-8 - PubMed

LinkOut - more resources

Feedback