The maleless protein associates with the X chromosome to regulate dosage compensation in Drosophila

Cell. 1991 Sep 6;66(5):935-47. doi: 10.1016/0092-8674(91)90439-6.

Abstract

The maleless (mle) gene is one of four known regulatory loci required for increased transcription (dosage compensation) of X-linked genes in D. melanogaster males. A predicted mle protein (MLE) contains seven short segments that define a superfamily of known and putative RNA and DNA helicases. MLE, while present in the nuclei of both male and female cells, differs in its association with polytene X chromosomes in the two sexes. MLE is associated with hundreds of discrete sites along the length of the X chromosome in males and not in females. The predominant localization of MLE to the X chromosome in males makes it a strong candidate to be a direct regulator of dosage compensation.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Blotting, Western
  • Cell Compartmentation
  • Chromosomal Proteins, Non-Histone*
  • Chromosome Mapping
  • Cloning, Molecular
  • DNA Helicases / genetics
  • DNA Helicases / immunology
  • DNA Helicases / metabolism
  • DNA-Binding Proteins*
  • Dosage Compensation, Genetic*
  • Drosophila Proteins*
  • Drosophila melanogaster / genetics*
  • Female
  • Fluorescent Antibody Technique
  • Gene Expression Regulation
  • Genes, Regulator*
  • Insect Hormones / genetics*
  • Insect Hormones / immunology
  • Insect Hormones / metabolism
  • Male
  • Molecular Sequence Data
  • Nuclear Proteins / genetics*
  • Nuclear Proteins / immunology
  • Nuclear Proteins / metabolism
  • RNA, Messenger / genetics
  • Transcription Factors / genetics*
  • Transcription Factors / immunology
  • Transcription Factors / metabolism
  • Transcription, Genetic
  • X Chromosome

Substances

  • Chromosomal Proteins, Non-Histone
  • DNA-Binding Proteins
  • Drosophila Proteins
  • Insect Hormones
  • Nuclear Proteins
  • RNA, Messenger
  • Transcription Factors
  • mle protein, Drosophila
  • DNA Helicases

Associated data

  • GENBANK/M60962
  • GENBANK/M64383
  • GENBANK/M64384
  • GENBANK/M74121
  • GENBANK/S44821
  • GENBANK/S54711
  • GENBANK/S55195
  • GENBANK/S55197
  • GENBANK/S56137
  • GENBANK/S56139