Papillomavirus E2 protein interacts with and stimulates human topoisomerase I

Virology. 2006 Apr 25;348(1):13-8. doi: 10.1016/j.virol.2006.02.018. Epub 2006 Mar 13.

Abstract

The papillomavirus (PV) E2 protein plays a role in recruiting viral and cellular DNA replication factors, such as PV E1 or RPA to PV genomes. Using both purified proteins and through co-precipitation, it was determined that HPV-11 E2 binds human topoisomerase I. E2 can stimulate topoisomerase I DNA relaxation activity 3- to 4-fold. Conversely, topoisomerase I is unable to stimulate E2 DNA binding. These findings suggest that stimulation of topoisomerase I by E2 may help promote efficient relaxation of the torsional stress induced by PV DNA replication.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • DNA Topoisomerases, Type I / metabolism*
  • DNA, Viral / metabolism
  • Electrophoretic Mobility Shift Assay
  • Enzyme-Linked Immunosorbent Assay
  • Immunoprecipitation
  • Plasmids / genetics
  • Protein Binding
  • Viral Proteins / metabolism*
  • Virus Replication / physiology

Substances

  • DNA, Viral
  • E2 protein, Human papillomavirus type 11
  • Viral Proteins
  • DNA Topoisomerases, Type I