Phagocytic functions and tumor necrosis factor secretion of human monocytes exposed to natural porcine surfactant (Curosurf)

Pediatr Res. 1991 Jul;30(1):69-74. doi: 10.1203/00006450-199107000-00015.


In this study we have analyzed various phagocytic functions and tumor necrosis factor (TNF) secretion of human monocytes exposed to either a biochemically well-defined porcine surfactant or a purified phospholipid preparation. Adherence, random migration, and chemotactic response to zymosan activated serum and formyl-methionyl-leucyl-phenylalanine were normal in surfactant-treated monocytes; surfactant was not a chemotactic stimulus. In contrast, phagocytosis of Staphylococcus aureus by monocytes exposed to surfactant (100 micrograms/mL) or phospholipids (100 micrograms/mL) was slightly impaired [surfactant: at 30 min (t30) 48.5 +/- 11%, t60 73.3 +/- 10.1%; phospholipids; t30 47.3 +/- 2.5%, t60 68.0 +/- 6.6%; controls: t30 66.6 +/- 9.9%, t60 81.0 +/- 6.6%, p less than 0.05 at t30 for both, p less than 0.05 at t60 for phospholipids]. Due to the smaller number of S. aureus ingested, bactericidal activity of surfactant- or phospholipid-treated monocytes was slightly reduced when compared with controls. Surfactant or phospholipids had no bactericidal activity. Uptake of Candida albicans was identical in surfactant- or phospholipid-treated monocytes and untreated controls; the same was true for the number of Candida organisms ingested per cell. Phagocytosis-associated chemiluminescence and production of superoxide anion by monocytes of either source in response to phorbol myristate acetate and opsonized zymosan were also unaffected.(ABSTRACT TRUNCATED AT 250 WORDS)

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Animals
  • Biological Products*
  • Blood Bactericidal Activity / drug effects
  • Cell Adhesion / drug effects
  • Chemotaxis, Leukocyte / drug effects
  • Humans
  • In Vitro Techniques
  • Monocytes / drug effects*
  • Monocytes / immunology
  • Monocytes / physiology
  • Phagocytosis / drug effects*
  • Phospholipids / pharmacology
  • Pulmonary Surfactants / pharmacology*
  • Staphylococcus aureus / immunology
  • Superoxides / metabolism
  • Swine
  • Tumor Necrosis Factor-alpha / metabolism*


  • Biological Products
  • Phospholipids
  • Pulmonary Surfactants
  • Tumor Necrosis Factor-alpha
  • Superoxides
  • poractant alfa