Condensin I interacts with the PARP-1-XRCC1 complex and functions in DNA single-strand break repair

Mol Cell. 2006 Mar 17;21(6):837-48. doi: 10.1016/j.molcel.2006.01.036.


Condensins are essential protein complexes critical for mitotic chromosome organization. Little is known about the function of condensins during interphase, particularly in mammalian cells. Here we report the interphase-specific interaction between condensin I and the DNA nick-sensor poly(ADP-ribose) polymerase 1 (PARP-1). We show that the association between condensin I, PARP-1, and the base excision repair (BER) factor XRCC1 increases dramatically upon single-strand break damage (SSB) induction. Damage-specific association of condensin I with the BER factors flap endonuclease 1 (FEN-1) and DNA polymerase delta/epsilon was also observed, suggesting that condensin I is recruited to interact with BER factors at damage sites. Consistent with this, DNA damage rapidly stimulates the chromatin association of PARP-1, condensin I, and XRCC1. Furthermore, depletion of condensin in vivo compromises SSB but not double-strand break (DSB) repair. Our results identify a SSB-specific response of condensin I through PARP-1 and demonstrate a role for condensin in SSB repair.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Adenosine Triphosphatases / metabolism
  • Adenosine Triphosphatases / physiology*
  • Animals
  • Animals, Genetically Modified
  • Carrier Proteins
  • Cell Cycle Proteins
  • Cell Line
  • Chickens / genetics
  • Chromatin
  • Chromosomal Proteins, Non-Histone
  • DNA Damage*
  • DNA Repair*
  • DNA, Single-Stranded
  • DNA-Binding Proteins / metabolism*
  • DNA-Binding Proteins / physiology*
  • HeLa Cells
  • Humans
  • Interphase
  • Mass Spectrometry
  • Mice / genetics
  • Mice, Knockout
  • Models, Biological
  • Multiprotein Complexes / metabolism
  • Multiprotein Complexes / physiology*
  • Nuclear Proteins
  • Poly(ADP-ribose) Polymerases / genetics*
  • Poly(ADP-ribose) Polymerases / metabolism
  • Poly(ADP-ribose) Polymerases / physiology
  • Transfection
  • X-ray Repair Cross Complementing Protein 1
  • Xenopus Proteins


  • Carrier Proteins
  • Cell Cycle Proteins
  • Chromatin
  • Chromosomal Proteins, Non-Histone
  • DNA, Single-Stranded
  • DNA-Binding Proteins
  • Multiprotein Complexes
  • Nuclear Proteins
  • SMC4 protein, Xenopus
  • X-ray Repair Cross Complementing Protein 1
  • XCAP-E protein, Xenopus
  • XRCC1 protein, human
  • Xenopus Proteins
  • Xrcc1 protein, mouse
  • condensin complexes
  • Poly(ADP-ribose) Polymerases
  • Adenosine Triphosphatases