Diffraction phase microscopy for quantifying cell structure and dynamics

Opt Lett. 2006 Mar 15;31(6):775-7. doi: 10.1364/ol.31.000775.

Abstract

We have developed diffraction phase microscopy as a new technique for quantitative phase imaging of biological structures. The method combines the principles of common path interferometry and single-shot phase imaging and is characterized by subnanometer path-length stability and millisecond-scale acquisition time. The potential of the technique for quantifying nanoscale motions in live cells is demonstrated by experiments on red blood cells.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Movement / physiology
  • Cells, Cultured
  • Equipment Design
  • Equipment Failure Analysis
  • Erythrocytes / cytology*
  • Erythrocytes / physiology
  • Humans
  • Image Enhancement / instrumentation*
  • Image Enhancement / methods
  • Image Interpretation, Computer-Assisted / instrumentation
  • Image Interpretation, Computer-Assisted / methods
  • Microscopy, Phase-Contrast / instrumentation*
  • Microscopy, Phase-Contrast / methods
  • Refractometry / instrumentation*
  • Refractometry / methods
  • Reproducibility of Results
  • Sensitivity and Specificity