Sensitivity of the Enzygnost anti-EBV/IgG for the determination of the Epstein-Barr virus immune status in pediatric patients

Diagn Microbiol Infect Dis. 2006 Jul;55(3):247-9. doi: 10.1016/j.diagmicrobio.2006.01.011. Epub 2006 Mar 20.


The Enzygnost Anti-EBV/IgG shows good performance for populations not defined by age. Samples from 349 pediatric outpatients were screened with the assay and with immunofluorescence assay (IFA) as the gold standard. The Enzygnost assay showed a sensitivity of 85%. Ten and one-tenth percent of EBV seropositive samples were classified as intermediate, and 4.9% were falsely determined as seronegative when compared to the standard IgG anti-virus capsid antigen (VCA) IFA. Our data suggest a limited sensitivity of the assay with samples from pediatric patients.

Publication types

  • Evaluation Study

MeSH terms

  • Adolescent
  • Antigens, Viral / immunology*
  • Capsid Proteins / immunology*
  • Child
  • Child, Preschool
  • Fluorescent Antibody Technique, Indirect
  • Herpesvirus 4, Human / immunology*
  • Humans
  • Immunoglobulin G / blood*
  • Infant
  • Mass Screening
  • Reagent Kits, Diagnostic / virology
  • Sensitivity and Specificity
  • Serologic Tests / methods


  • Antigens, Viral
  • Capsid Proteins
  • Epstein-Barr viral capsid antigen
  • Immunoglobulin G
  • Reagent Kits, Diagnostic