LTA recognition by bovine gammadelta T cells involves CD36

J Leukoc Biol. 2006 Jun;79(6):1268-70. doi: 10.1189/jlb.1005616. Epub 2006 Mar 21.


CD36 has recently been shown to facilitate monocyte Toll-like receptor 2 (TLR2) recognition of lipoteichoic acid (LTA), much like CD14 in TLR4 recognition of lipopolysaccharide. We previously found that bovine gammadelta T cells express CD36 transcripts. Here, we tested whether bovine gammadelta T cells express CD36 protein and if so, whether it functions in a manner similar to the monocyte molecule. CD36 transcripts and internal and cell surface protein could be detected in resting, sorted gammadelta T cells. Phorbol 12-myristate 13-acetate (PMA)/ionomycin treatment increased CD36 transcript levels (detectable at 4 h) and protein expression (internal and cell surface). Increased surface antigen expression was detectable by 24 h and was maximal at 72 h following PMA/ionomycin stimulation. Anti-CD36 monoclonal antibody inhibited increased macrophage-inflammatory protein-1alpha gene expression in gammadelta T cells activated by LTA. In conclusion, gammadelta T cells express CD36, previously thought to be a myeloid and endothelial cell-restricted surface antigen, and it contributes to responses by these cells to microbial LTA.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Antibodies, Monoclonal / immunology
  • Antibodies, Monoclonal / pharmacology
  • CD36 Antigens / immunology
  • CD36 Antigens / physiology*
  • Cattle / immunology*
  • Immunoglobulin M / immunology
  • Immunoglobulin M / pharmacology
  • Ionomycin / pharmacology
  • Lipopeptides
  • Lipopolysaccharides / pharmacology*
  • Peptides / pharmacology
  • Receptors, Antigen, T-Cell, gamma-delta / analysis*
  • T-Lymphocyte Subsets / drug effects
  • T-Lymphocyte Subsets / immunology*
  • Teichoic Acids / pharmacology*
  • Tetradecanoylphorbol Acetate / pharmacology
  • Toll-Like Receptor 2 / antagonists & inhibitors
  • Toll-Like Receptor 2 / physiology


  • Antibodies, Monoclonal
  • CD36 Antigens
  • Immunoglobulin M
  • Lipopeptides
  • Lipopolysaccharides
  • Pam(3)CSK(4) peptide
  • Peptides
  • Receptors, Antigen, T-Cell, gamma-delta
  • Teichoic Acids
  • Toll-Like Receptor 2
  • Ionomycin
  • lipoteichoic acid
  • Tetradecanoylphorbol Acetate