Mutation of phosphotransacetylase but not isocitrate lyase reduces the virulence of Salmonella enterica serovar Typhimurium in mice

Infect Immun. 2006 Apr;74(4):2498-502. doi: 10.1128/IAI.74.4.2498-2502.2006.


A phosphotransacetylase (pta) mutant of Salmonella enterica serovar Typhimurium was attenuated in mice but survived normally in macrophages. Complementation of the pta mutation in trans restored virulence. An isocitrate lyase (aceA) mutant was virulent, so the inability to use acetate as a sole carbon source does not explain the phenotype.

Publication types

  • Comparative Study
  • Research Support, N.I.H., Extramural

MeSH terms

  • Animals
  • Bacterial Proteins / genetics
  • Isocitrate Lyase / genetics*
  • Isocitrate Lyase / metabolism
  • Mice
  • Mice, Inbred BALB C
  • Mice, Inbred C3H
  • Mice, Inbred DBA
  • Mutation*
  • Phosphate Acetyltransferase / genetics*
  • Phosphate Acetyltransferase / metabolism
  • Salmonella Infections / microbiology
  • Salmonella Infections / mortality
  • Salmonella typhimurium / enzymology*
  • Salmonella typhimurium / genetics
  • Salmonella typhimurium / pathogenicity*
  • Virulence


  • Bacterial Proteins
  • Phosphate Acetyltransferase
  • Isocitrate Lyase