An immunostimulatory DNA sequence from a probiotic strain of Bifidobacterium longum inhibits IgE production in vitro

FEMS Immunol Med Microbiol. 2006 Apr;46(3):461-9. doi: 10.1111/j.1574-695X.2006.00064.x.


The immunostimulatory oligodeoxynucleotide (ODN) BL07 (5'-GCGTCGGTTTCGGTGCTCAC-3') was identified from the genomic DNA of the probiotic strain Bifidobacterium longum BB536. ODN BL07 stimulated B-lymphocyte proliferation and induced interleukin-12 (IL-12) production in macrophage-like J774.1 cells. ODNs BL07 and BL07S (modified with phosphorothioate backbone) significantly inhibited immunoglobulin E (IgE) production and stimulated interferon-gamma (IFN-gamma) and IL-12 production, but did not affect IL-4 secretion in murine splenic cells of ovalbumin-primed BALB/c mice. These ODNs also significantly inhibited production of IgE in purified murine B cells in the presence of IL-4 and anti-CD40. The results suggest the potential of ODNs BL07 and BL07S in preventing IgE-related immune responses and the possible involvement of ODN BL07 in the antiallergic efficacy of B. longum BB536.

MeSH terms

  • Animals
  • B-Lymphocytes / cytology
  • B-Lymphocytes / immunology
  • Base Sequence
  • Bifidobacterium / genetics*
  • Bifidobacterium / immunology*
  • Cell Proliferation
  • DNA, Bacterial / chemistry
  • DNA, Bacterial / genetics
  • DNA, Bacterial / immunology*
  • Enzyme-Linked Immunosorbent Assay
  • Immunoglobulin E / biosynthesis*
  • Immunoglobulin E / immunology
  • Immunoglobulin Isotypes / immunology
  • Interferon-gamma / immunology
  • Interleukin-12 / immunology
  • Interleukin-4 / immunology
  • Mice
  • Mice, Inbred BALB C
  • Molecular Sequence Data
  • NF-kappa B / immunology
  • Oligodeoxyribonucleotides / genetics*
  • Oligodeoxyribonucleotides / immunology
  • Oligodeoxyribonucleotides / isolation & purification
  • Probiotics / pharmacology*
  • Sequence Analysis, DNA
  • Specific Pathogen-Free Organisms


  • DNA, Bacterial
  • Immunoglobulin Isotypes
  • NF-kappa B
  • Oligodeoxyribonucleotides
  • Interleukin-12
  • Interleukin-4
  • Immunoglobulin E
  • Interferon-gamma