Alpha-melanocyte-stimulating hormone reduces interleukin-1 beta effects on rat stomach preparations possibly through interference with a type I receptor

Eur J Pharmacol. 1991 May 17;197(2-3):151-5. doi: 10.1016/0014-2999(91)90514-q.


Contractions elicited by CaCl2 on isolated rat stomach strip preparations have been reported to be potentiated by interleukin-1 beta (IL-1 beta). We have investigated whether this effect can be reduced by the putative IL-1 beta antagonist, alpha-melanocyte-stimulating hormone (alpha MSH). Additionally, the effects of alpha MSH on the specific binding of IL-1 beta to B- and T-cells have been investigated to further clarify its inhibitory activities. Both alpha MSH and its carboxyl terminal tripeptide concentration dependently reduced the potentiation of CaCl2-induced contractions caused by IL-1 beta but not those caused by leukotriene D4, the parent molecule being approximately 250 times more active. Additionally, both peptides potently and selectively reduced 125I-IL-1 beta binding to the T-cell sub-clone EL4-6.1 but not to the B-cell sub-clone 1H7. The results indicate that IL-1 beta effects on rat stomach may be mediated through a type-I (80 kDa) IL-1 beta receptor.

MeSH terms

  • Animals
  • Calcium Chloride / pharmacology
  • Clone Cells / drug effects
  • Clone Cells / metabolism
  • In Vitro Techniques
  • Interleukin-1 / antagonists & inhibitors*
  • Interleukin-1 / metabolism
  • Interleukin-1 / pharmacology
  • Male
  • Melanocyte-Stimulating Hormones / analogs & derivatives
  • Melanocyte-Stimulating Hormones / pharmacology
  • Muscle Contraction / drug effects
  • Peptide Fragments / pharmacology
  • Rats
  • Rats, Inbred Strains
  • Receptors, Immunologic / drug effects*
  • Receptors, Immunologic / physiology
  • Receptors, Interleukin-1
  • SRS-A / pharmacology
  • Stomach / drug effects*
  • Stomach / physiology
  • alpha-MSH / pharmacology*


  • Interleukin-1
  • Peptide Fragments
  • Receptors, Immunologic
  • Receptors, Interleukin-1
  • SRS-A
  • alpha-MSH
  • MSH (11-13)
  • Melanocyte-Stimulating Hormones
  • Calcium Chloride