Removal of contaminating TEM-la beta-lactamase gene from commercial Taq DNA polymerase

J Microbiol. 2006 Feb;44(1):126-8.


This study confirms that Taq DNA polymerase could be contaminated with the blaTEM-1(a) gene. It also proposes two different methods that could be used to overcome DNA contamination: (i) DNase I treatment prior to PCR amplification; and (ii) the use of a highly purified Taq DNA polymerase which was devoid of detectable contamination.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Decontamination / methods*
  • Deoxyribonucleases / metabolism
  • Equipment Contamination*
  • Polymerase Chain Reaction / methods*
  • Reagent Kits, Diagnostic*
  • Taq Polymerase / genetics*
  • Taq Polymerase / isolation & purification
  • beta-Lactamases / genetics*


  • Reagent Kits, Diagnostic
  • Taq Polymerase
  • Deoxyribonucleases
  • beta-Lactamases
  • beta-lactamase TEM-1