This site needs JavaScript to work properly. Please enable it to take advantage of the complete set of features!

Skip to main page content

Email citation

Add to Collections

Your saved search

Name of saved search:

Search terms:

Test search terms

Would you like email updates of new search results?

Yes
No

Email: (change)

Frequency:

Which day?

Which day?

Report format:

Send at most:

Send even when there aren't any new results

Optional text in email:

Your RSS Feed

. 2006 Apr;3(4):303-13.

doi: 10.1038/nmeth0406-303.

Optimization of membrane protein overexpression and purification using GFP fusions

David Drew¹, Mirjam Lerch, Edmund Kunji, Dirk-Jan Slotboom, Jan-Willem de Gier

Affiliations

PMID: 16554836
DOI: 10.1038/nmeth0406-303

Optimization of membrane protein overexpression and purification using GFP fusions

David Drew et al. Nat Methods. 2006 Apr.

. 2006 Apr;3(4):303-13.

doi: 10.1038/nmeth0406-303.

Authors

David Drew¹, Mirjam Lerch, Edmund Kunji, Dirk-Jan Slotboom, Jan-Willem de Gier

Affiliation

¹ Department of Biochemistry and Biophysics, Stockholm University, SE-106 91 Stockholm, Sweden.

PMID: 16554836
DOI: 10.1038/nmeth0406-303

No abstract available

PubMed Disclaimer

Similar articles

A scalable, GFP-based pipeline for membrane protein overexpression screening and purification.
Drew D, Slotboom DJ, Friso G, Reda T, Genevaux P, Rapp M, Meindl-Beinker NM, Lambert W, Lerch M, Daley DO, Van Wijk KJ, Hirst J, Kunji E, De Gier JW. Drew D, et al. Protein Sci. 2005 Aug;14(8):2011-7. doi: 10.1110/ps.051466205. Epub 2005 Jun 29. Protein Sci. 2005. PMID: 15987891 Free PMC article.
Application of split-green fluorescent protein for topology mapping membrane proteins in Escherichia coli.
Toddo S, Söderström B, Palombo I, von Heijne G, Nørholm MH, Daley DO. Toddo S, et al. Protein Sci. 2012 Oct;21(10):1571-6. doi: 10.1002/pro.2131. Epub 2012 Aug 21. Protein Sci. 2012. PMID: 22825803 Free PMC article.
Optimizing E. coli-based membrane protein production using Lemo21(DE3) and GFP-fusions.
Hjelm A, Schlegel S, Baumgarten T, Klepsch M, Wickström D, Drew D, de Gier JW. Hjelm A, et al. Methods Mol Biol. 2013;1033:381-400. doi: 10.1007/978-1-62703-487-6_24. Methods Mol Biol. 2013. PMID: 23996190
Large-scale production of membrane proteins in Saccharomyces cerevisiae: using a green fluorescent protein fusion strategy in the production of membrane proteins.
Drew D, Kim H. Drew D, et al. Methods Mol Biol. 2012;866:209-16. doi: 10.1007/978-1-61779-770-5_18. Methods Mol Biol. 2012. PMID: 22454126
Comparative Membrane Proteomics Reveals a Nonannotated E. coli Heat Shock Protein.
Yuan P, D'Lima NG, Slavoff SA. Yuan P, et al. Biochemistry. 2018 Jan 9;57(1):56-60. doi: 10.1021/acs.biochem.7b00864. Epub 2017 Oct 17. Biochemistry. 2018. PMID: 29039649 Free PMC article.

See all similar articles

Cited by

Fluorescent probe for high-throughput screening of membrane protein expression.
Backmark AE, Olivier N, Snijder A, Gordon E, Dekker N, Ferguson AD. Backmark AE, et al. Protein Sci. 2013 Aug;22(8):1124-32. doi: 10.1002/pro.2297. Epub 2013 Jul 3. Protein Sci. 2013. PMID: 23776061 Free PMC article.
Insights into outer membrane protein crystallization.
Newstead S, Hobbs J, Jordan D, Carpenter EP, Iwata S. Newstead S, et al. Mol Membr Biol. 2008 Dec;25(8):631-8. doi: 10.1080/09687680802526574. Mol Membr Biol. 2008. PMID: 19023694 Free PMC article.
GFP-based optimization scheme for the overexpression and purification of eukaryotic membrane proteins in Saccharomyces cerevisiae.
Drew D, Newstead S, Sonoda Y, Kim H, von Heijne G, Iwata S. Drew D, et al. Nat Protoc. 2008;3(5):784-98. doi: 10.1038/nprot.2008.44. Nat Protoc. 2008. PMID: 18451787 Free PMC article.
Saccharomyces cerevisiae-based platform for rapid production and evaluation of eukaryotic nutrient transporters and transceptors for biochemical studies and crystallography.
Scharff-Poulsen P, Pedersen PA. Scharff-Poulsen P, et al. PLoS One. 2013 Oct 4;8(10):e76851. doi: 10.1371/journal.pone.0076851. eCollection 2013. PLoS One. 2013. PMID: 24124599 Free PMC article.
Crystal structure of a bacterial homologue of the bile acid sodium symporter ASBT.
Hu NJ, Iwata S, Cameron AD, Drew D. Hu NJ, et al. Nature. 2011 Oct 5;478(7369):408-11. doi: 10.1038/nature10450. Nature. 2011. PMID: 21976025 Free PMC article.

See all "Cited by" articles

Publication types

Actions

MeSH terms

Actions
Actions
Actions
Actions
Actions
Actions
Actions
Actions
Actions
Actions
Actions
Actions
Actions

Substances

Actions
Actions
Actions
Actions
Actions

Grants and funding

MC_U105663139/MRC_/Medical Research Council/United Kingdom

LinkOut - more resources

Full Text Sources
- Nature Publishing Group
Other Literature Sources
- The Lens - Patent Citations Database