Optimization of membrane protein overexpression and purification using GFP fusions

Nat Methods. 2006 Apr;3(4):303-13. doi: 10.1038/nmeth0406-303.
No abstract available

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Escherichia coli Proteins / genetics
  • Escherichia coli Proteins / isolation & purification
  • Escherichia coli Proteins / metabolism
  • Gene Expression Regulation, Bacterial*
  • Green Fluorescent Proteins / chemistry*
  • Green Fluorescent Proteins / genetics
  • Green Fluorescent Proteins / metabolism
  • Luminescent Agents / pharmacology*
  • Membrane Proteins* / genetics
  • Membrane Proteins* / isolation & purification
  • Membrane Proteins* / metabolism
  • Recombinant Fusion Proteins / genetics
  • Recombinant Fusion Proteins / metabolism

Substances

  • Escherichia coli Proteins
  • Luminescent Agents
  • Membrane Proteins
  • Recombinant Fusion Proteins
  • Green Fluorescent Proteins