Isolation and characterization of a novel interferon-alpha-encoding gene, IFN-alpha 11, within a murine IFN cluster

Gene. 1991 Aug 15;104(2):187-95. doi: 10.1016/0378-1119(91)90249-b.


Two recombinant cosmids containing three complete murine interferon-alpha-encoding genes (Mu IFN-alpha) have been isolated from a mouse cosmid library. The cluster organization of these genes has been determined. A new Mu IFN-alpha gene (Mu IFN-alpha 11) has been isolated and studied with respect to its structure and inducible transcription pattern. The nucleotide and deduced amino acid sequences of the Mu IFN-alpha 11 gene, as compared to the two other IFN-alpha 7 and IFN-alpha 8 genes, show that, albeit highly homologous, these genes are all different. The transient expression of the three genes gave rise to proteins showing antiviral properties which were neutralized with murine anti-IFN-alpha antibodies. The transcription of the Mu IFN-alpha genes was studied in two uninduced or Newcastle-disease-virus-induced murine cell types. Mu IFN-alpha 11, as well as alpha 7 and alpha 8, are not expressed in L929 nor in C243 cells upon viral induction and therefore constitute an interesting model to study Mu IFN-alpha gene repression.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cell Line
  • Cloning, Molecular
  • DNA
  • Gene Expression
  • Interferon-alpha / genetics*
  • Interferon-alpha / physiology
  • Mice
  • Molecular Sequence Data
  • Multigene Family*
  • Restriction Mapping
  • Transcription, Genetic
  • Vesicular stomatitis Indiana virus / growth & development


  • Interferon-alpha
  • DNA

Associated data

  • GENBANK/M64117
  • GENBANK/M64118
  • GENBANK/M64119
  • GENBANK/M68944
  • GENBANK/S54966
  • GENBANK/S54968
  • GENBANK/S57214
  • GENBANK/S57438
  • GENBANK/S60812
  • GENBANK/X56411