Interleukin-4-dependent innate collaboration between iNKT cells and B-1 B cells controls adaptative contact sensitivity

Immunology. 2006 Apr;117(4):536-47. doi: 10.1111/j.1365-2567.2006.02330.x.


We showed that hepatic Valpha14+ invariant natural killer T (iNKT) cells, via their rapid interleukin (IL)-4 production, activate B-1 cells to initiate contact sensitivity (CS). This innate collaboration was absent in IL-4(-/-) and signal transducer and activator of transcription (STAT)-6(-/-) mice and was inhibited by anti-IL-4 treatment. These mice have defective CS because they fail to locally recruit the sensitized effector T cells of acquired immunity. Their CS is reconstituted by transfer of downstream-acting 1-day immune B-1 cells from wild-type mice. Responses were not reconstituted with B-1 cells from IL-4 receptor-alpha(-/-) or STAT-6(-/-) mice, nor by IL-4 treatment of B cell-deficient mice at immunization. Finally, IL-4 was preferentially and transiently produced by hepatic iNKT cells within 7 min after sensitization to mediate collaboration between innate-like iNKT cells and the B-1 B cells that participate in the recruitment of effector T cells in vivo.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adoptive Transfer
  • Animals
  • Antigens, Differentiation, B-Lymphocyte / analysis
  • B-Lymphocyte Subsets / immunology*
  • Cell Communication / immunology
  • Dermatitis, Contact / etiology
  • Dermatitis, Contact / immunology*
  • Female
  • Flow Cytometry
  • Histocompatibility Antigens Class II / analysis
  • Immune Tolerance / immunology
  • Interleukin-4 / biosynthesis
  • Interleukin-4 / immunology*
  • Killer Cells, Natural / immunology*
  • Liver / immunology
  • Lymphocyte Activation / immunology
  • Male
  • Mice
  • Mice, Inbred BALB C
  • Mice, Inbred CBA
  • Picryl Chloride
  • Receptors, Antigen, T-Cell, alpha-beta / analysis
  • Receptors, Interleukin-4 / immunology
  • STAT6 Transcription Factor / immunology
  • T-Lymphocyte Subsets / immunology*


  • Antigens, Differentiation, B-Lymphocyte
  • Histocompatibility Antigens Class II
  • Receptors, Antigen, T-Cell, alpha-beta
  • Receptors, Interleukin-4
  • STAT6 Transcription Factor
  • Stat6 protein, mouse
  • invariant chain
  • Interleukin-4
  • Picryl Chloride