Activation of BPV-1 replication in vitro by the transcription factor E2

Nature. 1991 Oct 17;353(6345):628-32. doi: 10.1038/353628a0.


Soluble extracts from uninfected murine cells supplemented with purified viral E1 and E2 proteins support the replication of exogenously added papilloma virus DNA. The E2 transactivator stimulates the binding of the E1 replication protein to the minimal origin of replication and activates DNA replication. These results support the concept that transcription factors have a direct role in the initiation of DNA replication in eukaryotes by participating in the assembly of a complex at the origin of replication.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Aphidicolin / pharmacology
  • Base Sequence
  • Binding Sites
  • Bovine papillomavirus 1 / physiology*
  • DNA Replication / drug effects*
  • DNA, Viral / biosynthesis
  • DNA, Viral / genetics
  • DNA, Viral / metabolism
  • Kinetics
  • Molecular Sequence Data
  • Regulatory Sequences, Nucleic Acid
  • Templates, Genetic
  • Transcription Factors / pharmacology*
  • Virus Replication / drug effects*


  • DNA, Viral
  • Transcription Factors
  • Aphidicolin