Abstract
A new pyridone alkaloid, (+)-N-deoxymilitarinone A (1), was isolated from Paecilomyces farinosus RCEF 0097 along with the related metabolites, militarinone D and militarinone B. The sterol 22E,4R-ergosta-7,22-diene-3beta,5alpha,6beta,9alpha-tetraol was also identified. The structures were established by spectroscopic methods, in particular with the aid of extensive NMR experiments. Compound 1 induced neurite sprouting in PC 12 cells when tested at 33 and 100 microM concentrations. A cytotoxic effect was observed in human neurons (IMR-32) at a concentration of 100 microM.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Alkaloids / chemistry
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Alkaloids / isolation & purification*
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Alkaloids / pharmacology
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Animals
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China
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Humans
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Lepidoptera
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Molecular Structure
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Nerve Growth Factors / chemistry
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Nerve Growth Factors / isolation & purification*
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Nerve Growth Factors / pharmacology
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Neurons / drug effects
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PC12 Cells
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Paecilomyces / chemistry*
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Pyridones / chemistry
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Pyridones / isolation & purification*
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Pyridones / pharmacology
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Rats
Substances
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(+)-N-deoxymilitarinone A
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Alkaloids
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Nerve Growth Factors
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Pyridones
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militarinone B
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militarinone D