Separation of 1,4-benzodiazepines by micellar elektrokinetic capillary chromatography

J Biochem Biophys Methods. 2007 Jan 10;69(3):251-9. doi: 10.1016/j.jbbm.2006.02.003. Epub 2006 Mar 9.


In this work the applicability of micellar elektrokinetic capillary chromatography (MECC) for the determination of benzodiazepines (BZD) has been studied. The applied method was used for the simultaneous separation of 8 BZDs (alprazolam, bromazepam, chlordiazepoxide, diazepam, flunitrazepam, medazepam, oxazepam, nitrazepam), and also for the study of stability in acidic medium. A fast and reliable method has been developed; using a separation buffer composed of sodium tetraborate 25 mM (pH 9.5), SDS (50 mM) and methanol (at least 12%) as an organic modifier.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Benzodiazepines / chemistry
  • Benzodiazepines / isolation & purification*
  • Borates / chemistry
  • Chromatography, Micellar Electrokinetic Capillary* / methods
  • Hydrogen-Ion Concentration
  • Methanol / chemistry
  • Sodium Dodecyl Sulfate / chemistry


  • Borates
  • Benzodiazepines
  • Sodium Dodecyl Sulfate
  • tetraborate
  • Methanol