Peripheral and central distribution of TRPV1, substance P and CGRP of rat corneal neurons

Brain Res. 2006 Apr 26;1085(1):87-94. doi: 10.1016/j.brainres.2006.02.035. Epub 2006 Mar 27.


The rat corneal neurons expressing vanilloid receptor TRPV1, substance P (SP) and calcitonin-gene-related peptide (CGRP) were examined. In the cornea, some TRPV1-immunoreactive nerve fibers displayed either SP- or CGRP immunoreactivity also. For observing corneal neuronal elements in the trigeminal ganglion (TG) and in the medulla oblongata, retrograde and anterograde cholera toxin subunit B (CTB) tracing methods combining with triple immunofluorescence technique were performed. The corneal neuronal somata were located in the ophthalmic division of the TG; 37% of them were immunoreactive for TRPV1. One third and three quarters of the corneal TRPV1-immunoreactive neurons co-expressed SP and CGRP, respectively. All of SP-immunoreactive corneal neurons exhibited TRPV1 immunoreactivity. They were predominantly medium-sized (mean +/- SE = 638.2 +/- 49.5 microm(2)) and significantly larger than SP-immunoreactive and TRPV1-immunonegative neurons in the ophthalmic division of the TG. The central projection fibers of corneal neurons co-expressing TRPV1 with SP and CGRP were observed at the subnucleus interpolaris/caudalis transition within trigeminal nucleus. The present study suggests that TRPV1 of the corneal neurons works in close relation to SP and CGRP both in the cornea and CNS for healing and nociceptive transduction.

Publication types

  • Comparative Study

MeSH terms

  • Animals
  • Calcitonin Gene-Related Peptide / metabolism*
  • Cell Size
  • Cholera Toxin / metabolism
  • Cornea / cytology*
  • Immunohistochemistry / methods
  • Medulla Oblongata / cytology*
  • Neurons / metabolism*
  • Rats
  • Rats, Sprague-Dawley
  • Substance P / metabolism*
  • TRPV Cation Channels / metabolism*
  • Trigeminal Ganglion / cytology*


  • TRPV Cation Channels
  • TRPV1 protein, mouse
  • Substance P
  • Cholera Toxin
  • Calcitonin Gene-Related Peptide