Multiplex PCR for genes encoding prevalent OXA carbapenemases in Acinetobacter spp

Int J Antimicrob Agents. 2006 Apr;27(4):351-3. doi: 10.1016/j.ijantimicag.2006.01.004. Epub 2006 Mar 24.


Carbapenem resistance in Acinetobacter baumannii is a growing public health concern and is most often mediated by OXA carbapenemases. We describe a novel multiplex polymerase chain reaction (PCR) assay able to detect and distinguish alleles encoding three subgroups of acquired OXA carbapenemases (OXA-23-like, OXA-24-like and OXA-58-like) that are scattered in Acinetobacter spp., and a fourth subgroup, OXA-51-like, which appears to be intrinsic to Acinetobacter baumannii. Isolates belonging to two prevalent UK A. baumannii 'OXA' clones (OXA-23 clones 1 and 2) had alleles encoding both an intrinsic OXA-51-like and an acquired OXA-23 enzyme, whereas isolates of the 'SE clone' had only an intrinsic bla(OXA-51-like) allele. Genes encoding OXA-58 were detected (with bla(OXA-51-like)) in a cluster of related isolates from a single hospital. This simple assay will assist in monitoring the mechanisms responsible for carbapenem resistance in Acinetobacter spp.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acinetobacter / enzymology
  • Acinetobacter / genetics*
  • Acinetobacter Infections / microbiology
  • Bacterial Proteins / genetics*
  • DNA, Bacterial / analysis
  • Drug Resistance, Bacterial / genetics*
  • Electrophoresis, Agar Gel
  • Humans
  • Microbial Sensitivity Tests
  • Polymerase Chain Reaction / methods*
  • United Kingdom
  • beta-Lactamases / genetics*


  • Bacterial Proteins
  • DNA, Bacterial
  • beta-lactamase OXA-23
  • beta-lactamase OXA-28
  • beta-Lactamases
  • beta-lactamase OXA-24
  • beta-lactamase OXA-58, Acinetobacter junii
  • carbapenemase