A method for production of antibodies to human T-cell receptor beta-chain variable regions

Proc Natl Acad Sci U S A. 1991 Oct 1;88(19):8357-61. doi: 10.1073/pnas.88.19.8357.

Abstract

Mouse T-cell hybridomas bearing human V beta elements were produced by transfection of human/mouse hybrid T-cell receptor beta-chain genes into a mouse T-cell hybridoma lacking an endogenous beta-chain gene. These hybridomas were entirely mouse in origin except for the human V beta region. These cells were used to immunize mice against human V beta elements. Mouse monoclonal antibodies have thus been generated against human V beta 13.1 and -13.2. We expect that the method outlined in this paper will be useful in the production of monoclonal antibodies specific for other human V beta or V alpha elements.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Antibodies, Monoclonal / immunology
  • Antibody Specificity
  • Base Sequence
  • Gene Rearrangement, beta-Chain T-Cell Antigen Receptor
  • Humans
  • Hybridomas
  • Mice
  • Molecular Sequence Data
  • Multigene Family
  • Oligonucleotides / chemistry
  • Polymerase Chain Reaction
  • Receptors, Antigen, T-Cell, alpha-beta / genetics
  • Receptors, Antigen, T-Cell, alpha-beta / immunology*
  • Recombinant Fusion Proteins / immunology
  • T-Lymphocytes / immunology*
  • Transfection

Substances

  • Antibodies, Monoclonal
  • Oligonucleotides
  • Receptors, Antigen, T-Cell, alpha-beta
  • Recombinant Fusion Proteins