Abstract
Mouse T-cell hybridomas bearing human V beta elements were produced by transfection of human/mouse hybrid T-cell receptor beta-chain genes into a mouse T-cell hybridoma lacking an endogenous beta-chain gene. These hybridomas were entirely mouse in origin except for the human V beta region. These cells were used to immunize mice against human V beta elements. Mouse monoclonal antibodies have thus been generated against human V beta 13.1 and -13.2. We expect that the method outlined in this paper will be useful in the production of monoclonal antibodies specific for other human V beta or V alpha elements.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Antibodies, Monoclonal / immunology
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Antibody Specificity
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Base Sequence
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Gene Rearrangement, beta-Chain T-Cell Antigen Receptor
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Humans
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Hybridomas
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Mice
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Molecular Sequence Data
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Multigene Family
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Oligonucleotides / chemistry
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Polymerase Chain Reaction
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Receptors, Antigen, T-Cell, alpha-beta / genetics
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Receptors, Antigen, T-Cell, alpha-beta / immunology*
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Recombinant Fusion Proteins / immunology
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T-Lymphocytes / immunology*
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Transfection
Substances
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Antibodies, Monoclonal
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Oligonucleotides
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Receptors, Antigen, T-Cell, alpha-beta
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Recombinant Fusion Proteins