Chemical modulation of receptor signaling inhibits regenerative angiogenesis in adult zebrafish

Nat Chem Biol. 2006 May;2(5):265-73. doi: 10.1038/nchembio778. Epub 2006 Mar 26.


We examined the role of angiogenesis and the need for receptor signaling using chemical inhibition of the vascular endothelial growth factor receptor in the adult zebrafish tail fin. Using a small-molecule inhibitor, we were able to exert precise control over blood vessel regeneration. An angiogenic limit to tissue regeneration was determined, as avascular tissue containing skin, pigment, neuronal axons and bone precursors could regenerate up to about 1 mm. This indicates that tissues can regenerate without direct interaction with endothelial cells and at a distance from blood supply. We also investigated whether the effects of chemical inhibition could be enhanced in zebrafish vascular mutants. We found that adult zebrafish, heterozygous for a mutation in the critical receptor effector phospholipase Cgamma1, show a greater sensitivity to chemical inhibition. This study illustrates the utility of the adult zebrafish as a new model system for receptor signaling and chemical biology.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Angiogenesis Inhibitors / pharmacology*
  • Animals
  • Axons / drug effects
  • Axons / physiology
  • Bone Regeneration / drug effects
  • Bone Regeneration / physiology
  • Endothelial Cells / drug effects*
  • Endothelial Cells / physiology
  • Guided Tissue Regeneration / methods
  • Heterozygote
  • Microscopy, Fluorescence
  • Mutation
  • Neurons / metabolism
  • Phospholipase C gamma / pharmacology
  • Pigment Epithelium of Eye / drug effects
  • Pigment Epithelium of Eye / physiology
  • Receptors, Vascular Endothelial Growth Factor / antagonists & inhibitors*
  • Receptors, Vascular Endothelial Growth Factor / metabolism
  • Regeneration / drug effects*
  • Regeneration / physiology
  • Skin Physiological Phenomena / drug effects
  • Tissue Engineering
  • Zebrafish / physiology*


  • Angiogenesis Inhibitors
  • Receptors, Vascular Endothelial Growth Factor
  • Phospholipase C gamma

Associated data

  • PubChem-Substance/10318716
  • PubChem-Substance/10318717
  • PubChem-Substance/10318718
  • PubChem-Substance/10318719