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, 77 (3), 96-103

[Purification and Study of Carbohydrate Specificity of Lectin From Sarcoscypha Coccinea (Fr.) Lambette]

[Article in Ukrainian]
  • PMID: 16566135

[Purification and Study of Carbohydrate Specificity of Lectin From Sarcoscypha Coccinea (Fr.) Lambette]

[Article in Ukrainian]
V O Antoniuk. Ukr Biokhim Zh (1999).

Abstract

A lectin that revealed affinity for lactose, N-acethylactosamine, 4-nitrophenyl-beta-D-gluco- and galactopyranosides from fruiting bodies of Sarcoscypha coccinea (Fr.) Lambette was purified by affinity chromatography on immobilized ovomucoid. According to electrophoresis data in 15% SDS-PAGE the lectin contains two very low-differing components with molecular weight 32 kDa. Molecular weight of the lectin is 128 kDa according to gel-chromatography on sephadex G-200. The lectin agglutinates rabbit erythrocytes and slightly weaker agglutinates human erythrocytes. After dialysis against 1% EDTA sodium salt solution the lectin loses hemaglutinating activity, but after the next dialysis against CaCl2 solution it is restored.

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