Application of lectin microarray to crude samples: differential glycan profiling of lec mutants

J Biochem. 2006 Mar;139(3):323-7. doi: 10.1093/jb/mvj070.


We recently developed a novel system for lectin microarray based on the evanescent-field fluorescence-detection principle, by which even weak lectin-oligosaccharide interactions are detectable without a washing procedure. For its practical application, cell glycan analysis was performed for Chinese hamster ovary (CHO) cells and their glycan profile was compared with those of their glycosylation-defective Lec mutants. Each of the cell surface extracts gave a significantly different profile from that of the parental CHO cells in a manner reflecting denoted biosynthetic features. Hence, the developed lectin microarray system is considered to be fully applicable for differential glycan profiling of crude samples.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • CHO Cells
  • Cricetinae
  • Cricetulus
  • Glycosylation
  • Lectins / chemistry
  • Lectins / genetics*
  • Lectins / metabolism
  • Mutation
  • Polysaccharides / chemistry*
  • Polysaccharides / metabolism
  • Protein Array Analysis*
  • Protein Binding


  • Lectins
  • Polysaccharides