The regulation of Saccharomyces cerevisiae FLO gene expression and Ca2+ -dependent flocculation by Flo8p and Mss11p

Curr Genet. 2006 Jun;49(6):375-83. doi: 10.1007/s00294-006-0068-z. Epub 2006 Mar 25.

Abstract

The ability of many microorganisms to modify adhesion-related properties of their cell surface is of importance for many processes, including substrate adhesion, cell-cell adhesion, invasive growth, pathogenic behaviour and biofilm formation. In the yeast Saccharomyces cerevisiae, a group of structurally related, cell-wall associated proteins encoded by the FLO gene family are directly responsible for many of the cellular adhesion phenotypes displayed by this organism. Previous research has suggested that the differential transcription of FLO genes determines specific adhesion phenotypes. However, the transcriptional regulation of most FLO genes remains poorly understood. Here we show that the transcriptional activator Mss11p, which has previously been shown to be involved in the regulation of starch degradation, the formation of pseudohyphae and haploid invasive growth, also acts as a strong inducer of flocculation. The data indicate that Mss11p induces flocculation together with Flo8p, and that FLO1 is the dominant target gene of the two factors in this process. The deletion of MSS11 leads to a non-flocculent phenotype, and specific domains of Mss11p that are critical for the induction of flocculation are identified. The data clearly show that several essential transcription factors are shared by at least two flocculation genes that control different adhesion phenotypes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Calcium / pharmacology*
  • Cell Adhesion
  • Flocculation
  • Gene Expression Regulation, Fungal / drug effects*
  • Gene Expression Regulation, Fungal / genetics*
  • Genes, Fungal / genetics
  • Mannose-Binding Lectins
  • Membrane Glycoproteins
  • Membrane Proteins / genetics
  • Nuclear Proteins / genetics*
  • Nuclear Proteins / metabolism
  • Phenotype
  • Plasmids
  • Saccharomyces cerevisiae / drug effects*
  • Saccharomyces cerevisiae / genetics*
  • Saccharomyces cerevisiae / physiology
  • Saccharomyces cerevisiae Proteins / genetics*
  • Saccharomyces cerevisiae Proteins / metabolism
  • Trans-Activators / genetics*
  • Trans-Activators / metabolism
  • Transcription Factors
  • Transcription, Genetic / drug effects
  • Transcription, Genetic / genetics

Substances

  • FLO1 protein, S cerevisiae
  • FLO11 protein, S cerevisiae
  • FLO8 protein, S cerevisaie
  • MSS11 protein, S cerevisiae
  • Mannose-Binding Lectins
  • Membrane Glycoproteins
  • Membrane Proteins
  • Nuclear Proteins
  • Saccharomyces cerevisiae Proteins
  • Trans-Activators
  • Transcription Factors
  • Calcium