Microtubule destabilization by cdc2/H1 histone kinase: phosphorylation of a "pro-rich region" in the microtubule-binding domain of MAP-4

Biochem Biophys Res Commun. 1991 Sep 30;179(3):1620-6. doi: 10.1016/0006-291x(91)91760-a.

Abstract

Microtubule-associated protein-4 (MAP-4), a major MAP in proliferating cells, consists of a microtubule-binding domain and a projection domain protruding from the microtubule wall. The former contains a Pro-rich region and an assembly-promoting (AP) sequence region which is common to the neuron-specific MAPs, MAP-2 and tau1. In this paper, we describe the phosphorylation of the Pro-rich region of MAP-4 and the suppression of its assembly-promoting activity by cdc2/H1 histone kinase. This inactivation of MAP-4 may cause disassembly of the interphase microtubular network at the end of the G2 phase of the cell cycle.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • CDC2 Protein Kinase / metabolism*
  • Cell Line
  • Kinetics
  • Mammary Neoplasms, Experimental
  • Mice
  • Microtubules / metabolism*
  • Molecular Sequence Data
  • Oocytes / physiology
  • Peptides / chemical synthesis
  • Phosphorylation
  • Protamine Kinase / metabolism*
  • Xenopus

Substances

  • Peptides
  • Protamine Kinase
  • CDC2 Protein Kinase