Shaggy/GSK-3beta kinase localizes to the centrosome and to specialized cytoskeletal structures in Drosophila

Cell Motil Cytoskeleton. 2006 Jun;63(6):313-20. doi: 10.1002/cm.20128.

Abstract

The assembly of a functional bipolar mitotic spindle requires an exquisite regulation of microtubule behavior in time and space. To characterize new elements of this machinery we carried out a GFP based "protein trap" screen and selected fusion proteins which localized to the spindle apparatus. By this method we identified Shaggy, the Drosophila homologue of glycogen synthase kinase-3beta (GSK-3beta), as a component of centrosomes. GSK-3beta acting in the Wingless signaling pathway is involved in a vast range of developmental processes, from pattern formation to cell-fate specification, and is a key factor for cell proliferation in most animals. We exploited our Shaggy::GFP Drosophila line to analyze the subcellular localizations of GSK-3beta/Shaggy and shed light on its multiple roles during embryogenesis. We found that Shaggy becomes enriched transiently in a variety of specialized cytoskeletal structures of the embryo, including centrosomes throughout mitosis, suggesting that this kinase is involved in the regulation of many aspects of the cytoskeleton function.

MeSH terms

  • Animals
  • Cell Proliferation
  • Centrosome / enzymology*
  • Centrosome / physiology
  • Centrosome / ultrastructure
  • Cytoskeleton / enzymology*
  • Cytoskeleton / physiology
  • Cytoskeleton / ultrastructure
  • Drosophila / embryology
  • Drosophila / enzymology*
  • Drosophila / genetics
  • Drosophila / ultrastructure
  • Drosophila Proteins / metabolism*
  • Glycogen Synthase Kinase 3 / metabolism*
  • Green Fluorescent Proteins / biosynthesis
  • Green Fluorescent Proteins / genetics
  • Green Fluorescent Proteins / metabolism
  • Proto-Oncogene Proteins / metabolism
  • Spindle Apparatus / enzymology*
  • Spindle Apparatus / physiology
  • Spindle Apparatus / ultrastructure
  • Wnt1 Protein

Substances

  • Drosophila Proteins
  • Proto-Oncogene Proteins
  • Wnt1 Protein
  • wg protein, Drosophila
  • Green Fluorescent Proteins
  • Sgg protein, Drosophila
  • Glycogen Synthase Kinase 3