Identification of the basement membrane protein nidogen as a candidate ligand for tumor endothelial marker 7 in vitro and in vivo

FEBS Lett. 2006 Apr 17;580(9):2253-7. doi: 10.1016/j.febslet.2006.03.033. Epub 2006 Mar 20.

Abstract

Tumor endothelial marker 7 (TEM7) is a novel transmembrane protein that is highly expressed in the tumor endothelium. However, the ligands and functions of TEM7 are unknown at present. Using a recombinant ectodomain of TEM7 as a probe, we could identify the saturable interaction between nidogen and TEM7 in vitro. A cell overlay binding assay showed that recombinant nidogen interact with full length TEM7 on cell surface. Finally, nidogen/TEM7 interaction enhanced cell spreading in TEM7 transfected 293T cells. This novel nidogen/TEM7 interaction may provide an important molecular tool with potential therapeutic applications in the anti-angiogenic therapies.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biomarkers, Tumor / genetics*
  • Biomarkers, Tumor / metabolism
  • Cell Adhesion / genetics
  • Cell Line
  • Humans
  • Ligands
  • Membrane Glycoproteins / genetics*
  • Membrane Glycoproteins / metabolism
  • Neoplasm Proteins / genetics*
  • Neoplasm Proteins / metabolism
  • Neoplasms / blood supply
  • Neoplasms / genetics*
  • Neoplasms / metabolism
  • Protein Binding
  • Protein Structure, Tertiary / genetics
  • Receptors, Cell Surface / genetics*
  • Receptors, Cell Surface / metabolism

Substances

  • Biomarkers, Tumor
  • Ligands
  • Membrane Glycoproteins
  • Neoplasm Proteins
  • PLXDC1 protein, human
  • Receptors, Cell Surface
  • nidogen