A novel P-type ATPase from yeast involved in sodium transport

FEBS Lett. 1991 Oct 21;291(2):189-91. doi: 10.1016/0014-5793(91)81280-l.


The gene ENA1 was cloned by its ability to complement the Li+ sensitivity of a low Li(+)-efflux strain. The nucleotide sequence of the cloned DNA fragment showed that there are two almost identical genes in tandem, and predicts that they encode P-ATPases. Disruption of both genes originated a strain defective in Na+ and Li+ effluxes, and sensitive to Na+, to Li+ and to alkaline pH. By transformation with ENA1 the defective effluxes and tolerances were repaired.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biological Transport, Active
  • Hydrogen-Ion Concentration
  • Lithium / metabolism
  • Phosphoproteins / metabolism
  • Phosphoproteins / physiology
  • Potassium / metabolism
  • Saccharomyces cerevisiae / enzymology*
  • Saccharomyces cerevisiae / genetics
  • Saccharomyces cerevisiae / growth & development
  • Sodium-Potassium-Exchanging ATPase / classification
  • Sodium-Potassium-Exchanging ATPase / metabolism*
  • Sodium-Potassium-Exchanging ATPase / physiology*
  • Transformation, Genetic


  • Phosphoproteins
  • Lithium
  • Sodium-Potassium-Exchanging ATPase
  • Potassium