Cloning and tissue-specific expression of the brain calcium channel beta-subunit

FEBS Lett. 1991 Oct 21;291(2):253-8. doi: 10.1016/0014-5793(91)81296-k.


A cDNA clone encoding a protein with high homology to the beta-subunit of the rabbit skeletal muscle dihydropyridine-sensitive calcium channel was isolated from a rat brain cDNA library. This rat brain beta-subunit cDNA hybridizes to a 3.4 kb message that is expressed in high levels in the cerebral hemispheres and hippocampus but is significantly reduced in cerebellum. The open reading frame encodes 597 amino acids with a predicted mass of 65 679 Da which is 82% homologous with the skeletal muscle beta-subunit. The brain cDNA encodes a unique 153 amino acid C-terminus and predicts the absence of a muscle-specific 50 amino acid internal segment. It also encodes numerous consensus phosphorylation sites suggesting a role in calcium channel regulation. The corresponding human beta-subunit gene was localized to chromosome 17. Hence the encoded brain beta-subunit, which has a primary structure highly similar to its isoform in skeletal muscle, may have a comparable role as an integral regulatory component of a neuronal calcium channel.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Brain Chemistry*
  • Calcium Channels / metabolism*
  • Cloning, Molecular
  • Cricetinae
  • Dihydropyridines / metabolism
  • Gene Expression
  • Humans
  • Molecular Sequence Data
  • Rabbits
  • Rats
  • Receptors, Nicotinic / genetics*
  • Receptors, Nicotinic / isolation & purification
  • Sequence Homology, Nucleic Acid
  • Sheep


  • Calcium Channels
  • Dihydropyridines
  • Receptors, Nicotinic
  • 1,4-dihydropyridine

Associated data

  • GENBANK/X61394