Relationships between the level of matrix metalloproteinase-2 and tumor size of breast cancer

Clin Chim Acta. 2006 Sep;371(1-2):92-6. doi: 10.1016/j.cca.2006.02.026. Epub 2006 Feb 28.


Background: The involvements of matrix metalloproteinase-2 (MMP-2) in the pathogenesis of breast cancers have been established. We determined the concentrations of MMP-2 in serum samples and tumor tissues of breast cancer patients.

Methods: Gelatin zymography and ELISA were used to measure MMP-2 and MMP-9 concentrations in 90 breast cancer patients, including 60 tissue samples and 30 serum samples.

Results: ProMMP-2, activated MMP-2, proMMP-9 and activated MMP-9 levels were significantly higher in tumor tissues than that of corresponding paired adjacent normal tissue of 60 breast cancer patients (p<0.01). Further linear regression analysis has showed that the tumor size positively correlated with MMP-2 level in tumor tissue samples (R=0.55, p<0.0001), as well as with that of in serum samples (R=0.398, p=0.032). In addition, further statistical analysis for clinic pathological parameters revealed that MMP-2 level was significantly increased in patients with metastasis (p<0.05). Furthermore, MMP-2 level was significantly different between tumor grades (p=0.006).

Conclusions: MMP-2 levels in serum and tumor tissue might reflect the severity of invasion of breast cancer and various MMP inhibitors might be selectively used as potential anti-metastasis agents according to tumor size.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biomarkers, Tumor / analysis*
  • Breast Neoplasms / blood
  • Breast Neoplasms / enzymology*
  • Breast Neoplasms / pathology*
  • Disease Progression
  • Enzyme-Linked Immunosorbent Assay
  • Gelatin / metabolism
  • Humans
  • Matrix Metalloproteinase 2 / metabolism*
  • Neoplasm Invasiveness / pathology*
  • Neoplasm Staging*
  • Regression Analysis
  • Tissue Inhibitor of Metalloproteinase-2 / physiology


  • Biomarkers, Tumor
  • Tissue Inhibitor of Metalloproteinase-2
  • Gelatin
  • Matrix Metalloproteinase 2