Expression of human DEC-205 (CD205) multilectin receptor on leukocytes

Int Immunol. 2006 Jun;18(6):857-69. doi: 10.1093/intimm/dxl022. Epub 2006 Mar 31.


DEC-205 (CD205) belongs to the macrophage mannose receptor family of C-type lectin endocytic receptors and behaves as an antigen uptake/processing receptor for dendritic cells (DC). To investigate DEC-205 tissue distribution in human leukocytes, we generated a series of anti-human DEC-205 monoclonal antibodies (MMRI-5, 6 and 7), which recognized epitopes within the C-type lectin-like domains 1 and 2, and the MMRI-7 immunoprecipitated a single approximately 200 kDa band, identified as DEC-205 by mass spectrometry. MMRI-7 and another DEC-205 mAb (MG38), which recognized the epitope within the DEC-205 cysteine-rich and fibronectin type II domain, were used to examine DEC-205 expression by human leukocytes. Unlike mouse DEC-205, which is reported to have predominant expression on DC, human DEC-205 was detected by flow cytometry at relatively high levels on myeloid blood DC and monocytes, at moderate levels on B lymphocytes and at low levels on NK cells, plasmacytoid blood DC and T lymphocytes. MMRI-7 F(ab')2 also labeled monocytes, B lymphocytes and NK cells similarly excluding reactivity due to non-specific binding of the mAb to FcgammaR. Tonsil mononuclear cells showed a similar distribution of DEC-205 staining on the leukocytes. DEC-205-specific semiquantitative immunoprecipitation/western blot and quantitative reverse transcriptase-PCR analysis established that these leukocyte populations expressed DEC-205 protein and the cognate mRNA. Thus, human DEC-205 is expressed on more leukocyte populations than that were previously assumed based on mouse DEC-205 tissue localization studies. The broader DEC-205 tissue expression in man is relevant to clinical DC targeting strategies and DEC-205 functional studies.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Monoclonal / immunology
  • Antibodies, Monoclonal / pharmacology
  • Antigen Presentation* / immunology
  • Antigens, CD / biosynthesis*
  • Antigens, CD / immunology
  • Cell Line
  • Dendritic Cells / immunology
  • Dendritic Cells / metabolism*
  • Endocytosis / immunology
  • Gene Expression Regulation*
  • Humans
  • Lectins, C-Type / biosynthesis*
  • Lectins, C-Type / immunology
  • Leukocytes / immunology
  • Leukocytes / metabolism*
  • Mannose-Binding Lectins / biosynthesis
  • Mannose-Binding Lectins / immunology
  • Mice
  • Minor Histocompatibility Antigens
  • Organ Specificity
  • Receptors, Cell Surface / biosynthesis*
  • Receptors, Cell Surface / immunology


  • Antibodies, Monoclonal
  • Antigens, CD
  • DEC-205 receptor
  • Lectins, C-Type
  • Mannose-Binding Lectins
  • Minor Histocompatibility Antigens
  • Receptors, Cell Surface
  • mannose receptor