Anti-inflammatory mechanisms of the Tibetan herbal preparation Padma 28 in the vessel wall

Forsch Komplementmed. 2006 Feb;13 Suppl 1:13-7. doi: 10.1159/000090435. Epub 2006 Feb 17.

Abstract

Background: The Tibetan herbal preparation Padma 28 has been shown to act as an anti-atherosclerotic agent in advanced peripheral arterial occlusive disease. We tested the effect of aqueous Padma 28 extracts on both the Creactive protein (CRP) induced expression of the pro-inflammatory cell adhesion molecule E-selectin and the anti-atherosclerotic protective enzyme heme oxygenase- 1 (HO-1) in human aortic endothelial cells.

Methods and results: According to FACS analysis, quantitative RT-PCR and Western blot, CRP-induced E-selectin expression was completely prevented by aqueous Padma 28 extracts. Additionally, Padma 28 mediated an up to 60-fold upregulation of HO-1 mRNA as measured by quantitative RT-PCR. This upregulation could also be demonstrated on the protein level.

Conclusion: Aqueous extracts of the Tibetan herbal preparation Padma 28 inhibit CRP-induced expression of the inflammatory cell adhesion molecule E-selectin and lead to upregulation of the vascular protective enzyme HO-1 in human aortic endothelial cells. These properties may be responsible for its anti-atherosclerotic effects in peripheral arterial occlusive disease.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anti-Inflammatory Agents / therapeutic use*
  • Arteriosclerosis / drug therapy*
  • Blood Vessels / drug effects
  • Blood Vessels / enzymology
  • Blood Vessels / metabolism
  • C-Reactive Protein / analysis
  • C-Reactive Protein / antagonists & inhibitors
  • Cells, Cultured
  • E-Selectin / drug effects
  • Heme Oxygenase-1 / metabolism
  • Humans
  • Medicine, Tibetan Traditional / methods*
  • Phytotherapy*
  • Plant Extracts / therapeutic use*
  • Treatment Outcome

Substances

  • Anti-Inflammatory Agents
  • E-Selectin
  • Plant Extracts
  • Padma 28
  • C-Reactive Protein
  • Heme Oxygenase-1