The first immunoglobulin-like domain of porcine nectin-1 is sufficient to confer resistance to pseudorabies virus infection in transgenic mice

Arch Virol. 2006 Sep;151(9):1827-39. doi: 10.1007/s00705-006-0747-6. Epub 2006 Apr 3.


Nectin-1 is an alphaherpesvirus receptor that binds to virion glycoprotein D (gD). Porcine nectin-1 mediates entry of pseudorabies virus (PRV), herpes simplex virus types 1 and 2 (HSV-1 and HSV-2), and bovine herpesvirus type 1 (BHV-1). The gD-binding domain of nectin-1 is the first or N-terminal immunoglobulin (Ig)-like domain of the entire ectodomain. Here, we generated three transgenic mouse lines expressing a fusion protein consisting of the first Ig-like domain of porcine nectin-1 and the Fc portion of porcine IgG1 to assess the antiviral potential of the first Ig-like domain of nectin-1 in vivo. All of the transgenic mouse lines showed significant resistance to PRV infection via intraperitoneal inoculation (survival rates of 67% to 100%). In the intranasal challenge, a lower but still significant protection was observed; 21% to 55% of the animals from the three transgenic mouse lines survived. The present results demonstrate that a soluble form of the first domain of porcine nectin-1 is able to exert a significant antiviral effect against pseudorabies virus infection.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Adhesion Molecules / chemistry
  • Cell Adhesion Molecules / physiology*
  • Disease Models, Animal
  • Herpesvirus 1, Suid / growth & development*
  • Immunity, Innate / genetics*
  • Immunoglobulin Fc Fragments / genetics
  • Immunoglobulin G / genetics
  • Mice
  • Mice, Inbred C57BL
  • Mice, Transgenic
  • Nectins
  • Protein Structure, Tertiary
  • Pseudorabies / genetics
  • Pseudorabies / immunology
  • Pseudorabies / virology*
  • Receptors, Virus / chemistry
  • Receptors, Virus / physiology*
  • Recombinant Fusion Proteins / chemistry
  • Survival Analysis


  • Cell Adhesion Molecules
  • Immunoglobulin Fc Fragments
  • Immunoglobulin G
  • Nectin1 protein, mouse
  • Nectins
  • Receptors, Virus
  • Recombinant Fusion Proteins