The in vitro effect of different PRP concentrations on osteoblasts and fibroblasts

Clin Oral Implants Res. 2006 Apr;17(2):212-9. doi: 10.1111/j.1600-0501.2005.01203.x.

Abstract

Objectives: The aim of this study was to assess the biological rationale for the use of platelet-rich plasma (PRP) by evaluating the effect of different concentrations of PRP on osteoblasts (OB) and fibroblasts (FB) function in vitro.

Material and methods: PRP was obtained from volunteer donors using standard protocols. Primary human cultures of oral FBs and OBs were exposed to both activated and non-activated plasma as well as various concentrations of PRP (2.5 x, 3.5 x and max (4.2-5.5 x)). Cell proliferation was evaluated after 24 and 72 h using an MTT proliferation assay. Production of osteocalcin (OCN), osteoprotegerin (OPG) and transforming growth factor beta1 (TGF-beta1) was evaluated in OB after 24 and 72 h. Statistical analysis was performed using one-way ANOVA.

Results: PRP-stimulated cell proliferation in both OBs and FBs. The effect of different PRP concentrations on cell proliferation was most notable at 72 h. The maximum effect was achieved with a concentration of 2.5 x, with higher concentrations resulting in a reduction of cell proliferation. Upregulation of OCN levels and downregulation of OPG levels were noted with increasing PRP concentrations at both 24 and 72 h. TGF-beta1 levels were stimulated by increasing concentrations of PRP, with the increased levels being maintained at 72 h.

Conclusions: PRP preparations exert a dose-specific effect on oral FBs and OBs. Optimal results were observed at a platelet concentration of 2.5 x, which was approximately half of the maximal concentrate that could be obtained. Increased concentrations resulted in a reduction in proliferation and a suboptimal effect on OB function. Hence, different PRP concentrations may have an impact on the results that can be obtained in vivo.

MeSH terms

  • Adult
  • Blood Platelets / physiology*
  • Bone and Bones
  • Cell Proliferation
  • Cells, Cultured
  • Down-Regulation
  • Female
  • Fibroblasts / physiology*
  • Gingiva
  • Glycoproteins / analysis
  • Humans
  • Middle Aged
  • Osteoblasts / physiology*
  • Osteocalcin / analysis
  • Osteoprotegerin
  • Plasma
  • Receptors, Cytoplasmic and Nuclear / analysis
  • Receptors, Tumor Necrosis Factor / analysis
  • Time Factors
  • Transforming Growth Factor beta / analysis
  • Transforming Growth Factor beta1
  • Up-Regulation

Substances

  • Glycoproteins
  • Osteoprotegerin
  • Receptors, Cytoplasmic and Nuclear
  • Receptors, Tumor Necrosis Factor
  • TGFB1 protein, human
  • TNFRSF11B protein, human
  • Transforming Growth Factor beta
  • Transforming Growth Factor beta1
  • Osteocalcin