An allele of gyrA prevents Salmonella enterica serovar Typhimurium from using succinate as a carbon source

J Bacteriol. 2006 Apr;188(8):3126-9. doi: 10.1128/JB.188.8.3126-3129.2006.


A mutant gyrA allele resulting in an A271E substitution in the DNA gyrase protein generated a strain unable to grow on the C(4)-dicarboxylates succinate, malate, and fumarate. Bacteria harboring gyrA751 displayed decreased negative supercoiling in cells. Expression of the dctA gene, which encodes the C(4)-dicarboxylate transporter, was reduced in a gyrA751 mutant, providing the first evidence that dctA expression is supercoiling sensitive and uncovering a simple metabolic screen for lesions in gyrase that reduce negative supercoiling.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alleles
  • Amino Acid Substitution
  • Carbon / metabolism*
  • DNA Gyrase / genetics*
  • DNA, Superhelical / genetics
  • Fumarates / metabolism
  • Gene Expression
  • Malates / metabolism
  • Membrane Transport Proteins / genetics
  • Mutation, Missense
  • Salmonella typhimurium / genetics*
  • Salmonella typhimurium / growth & development
  • Salmonella typhimurium / metabolism*
  • Succinic Acid / metabolism*


  • DNA, Superhelical
  • Fumarates
  • Malates
  • Membrane Transport Proteins
  • Carbon
  • malic acid
  • fumaric acid
  • Succinic Acid
  • DNA Gyrase