Changes in the rat heart proteome induced by exercise training: Increased abundance of heat shock protein hsp20

Proteomics. 2006 May;6(10):3154-69. doi: 10.1002/pmic.200401356.


Chronic exercise training elicits adaptations in the heart that improve pump function and confer cardioprotection. To identify molecular mechanisms by which exercise training stimulates this favorable phenotype, a proteomic approach was employed to detect rat cardiac proteins that were differentially expressed or modified after exercise training. Exercise-trained rats underwent six weeks of progressive treadmill training five days/week, 0% grade, using an interval training protocol. Sedentary control rats were age- and weight-matched to the exercise-trained rats. Hearts were harvested at various times (0-72 h) after the last bout of exercise and were used to generate 2-D electrophoretic proteome maps and immunoblots. Compared with hearts of sedentary rats, 26 protein spot intensities were significantly altered in hypertrophied hearts of exercise-trained rats (p <0.05), and 12 spots appeared exclusively on gels from hearts of exercise-trained rats. Immunoblotting confirmed that chronic exercise training, but not a single bout of exercise, elicited a 2.5-fold increase in the abundance of one of the candidate proteins in the heart, a 20 kDa heat shock protein (hsp20) that persisted for at least 72 h of detraining. Thus, exercise training alters the cardiac proteome of the rat heart; the changes include a marked increase in the expression of hsp20.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Body Weight
  • Electrophoresis, Gel, Two-Dimensional
  • Female
  • HSP20 Heat-Shock Proteins / biosynthesis*
  • Heart / anatomy & histology
  • Immunoblotting
  • Mass Spectrometry
  • Muscle Proteins / biosynthesis*
  • Myocardium / metabolism*
  • Organ Size
  • Phosphorylation
  • Physical Conditioning, Animal*
  • Proteome / biosynthesis*
  • Rats
  • Rats, Sprague-Dawley
  • Rats, Wistar


  • HSP20 Heat-Shock Proteins
  • Muscle Proteins
  • Proteome
  • Hspb6 protein, rat