Expression cloning of an adenylate cyclase-coupled calcitonin receptor

Science. 1991 Nov 15;254(5034):1022-4. doi: 10.1126/science.1658940.


A calcitonin receptor complementary DNA (cDNA) was cloned by expression of a cDNA library from a porcine kidney epithelial cell line in COS cells. The 482-amino acid receptor has high affinity for salmon calcitonin (dissociation constant Kd approximately 6 nM) and is functionally coupled to increases in intracellular cyclic adenosine monophosphate (cAMP). The receptor shows no sequence similarity to other reported G protein-coupled receptors but is homologous to the parathyroid hormone-parathyroid hormone-related peptide (PTH-PTHrP) receptor, indicating that the receptors for these hormones, which regulate calcium homeostasis, represent a new family of G protein-coupled receptors.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adenylyl Cyclases / physiology
  • Amino Acid Sequence
  • Animals
  • Blotting, Northern
  • Calcitonin / metabolism*
  • Cloning, Molecular
  • Cyclic AMP / physiology
  • DNA / genetics
  • Gene Expression
  • Kidney / physiology
  • Molecular Sequence Data
  • RNA, Messenger / genetics
  • Receptors, Calcitonin
  • Receptors, Cell Surface / genetics*
  • Swine


  • RNA, Messenger
  • Receptors, Calcitonin
  • Receptors, Cell Surface
  • Calcitonin
  • DNA
  • Cyclic AMP
  • Adenylyl Cyclases

Associated data

  • GENBANK/M63875
  • GENBANK/M65143
  • GENBANK/M74420
  • GENBANK/M74445
  • GENBANK/M74517
  • GENBANK/M76239
  • GENBANK/M76240
  • GENBANK/M76241
  • GENBANK/M76242
  • GENBANK/M76243
  • GENBANK/M76244