Skip to main page content
Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1984 Oct;81(19):6024-8.
doi: 10.1073/pnas.81.19.6024.

Cloned Avirulence Gene of Pseudomonas Syringae Pv. Glycinea Determines Race-Specific Incompatibility on Glycine Max (L.) Merr

Affiliations
Free PMC article

Cloned Avirulence Gene of Pseudomonas Syringae Pv. Glycinea Determines Race-Specific Incompatibility on Glycine Max (L.) Merr

B J Staskawicz et al. Proc Natl Acad Sci U S A. .
Free PMC article

Abstract

A genomic library of Pseudomonas syringae pv. glycinea race 6 DNA was constructed in the mobilizable cosmid vector pLAFR1 and maintained in Escherichia coli HB101. Completeness of the library was estimated by assaying clones for the expression of ice-nucleating activity in E. coli. Ice-nucleation activity was represented approximately once in every 600 clones. Six hundred eighty random race 6 cosmid clones were mobilized from E. coli by plasmid pRK2013 in individual conjugations to a race 5 strain of P. s. glycinea. A single clone (pPg6L3) was detected that changed the race specificity of race 5 from virulent (compatible) to avirulent (incompatible) on the appropriate soybean cultivars. The clone was also mobilized from E. coli into race 1 and race 4 strains of P. s. glycinea, and it conferred on these transconjugants the same host range incompatibility as the wild-type race 6 strain. The cosmid clone was mapped by restriction endonucleases, and two adjacent EcoRI fragments were identified by transposon Tn5 mutagenesis to be important in determining race specificity. Southern blot analysis showed that the two EcoRI fragments are unique to race 6 and are not present in the other races tested. The cosmid clone pPg6L3 was also mobilized to Pseudomonas fluorescens and Rhizobium japonicum. However, neither these isolates nor E. coli harboring pPg6L3 elicited a hypersensitive reaction in soybean leaves.

Similar articles

See all similar articles

Cited by 95 articles

See all "Cited by" articles

References

    1. Appl Environ Microbiol. 1978 Dec;36(6):831-8 - PubMed
    1. Nature. 1981 Jan 1;289(5793):85-8 - PubMed
    1. Gene. 1982 Jun;18(3):289-96 - PubMed
    1. Proc Natl Acad Sci U S A. 1980 Dec;77(12):7347-51 - PubMed
    1. J Lab Clin Med. 1954 Aug;44(2):301-7 - PubMed

LinkOut - more resources

Feedback