Cauliflower mosaic virus coat protein is phosphorylated in vitro by a virion-associated protein kinase

J Martinez-Izquierdo; T Hohn

doi:10.1073/pnas.84.7.1824

Cauliflower mosaic virus coat protein is phosphorylated in vitro by a virion-associated protein kinase

Proc Natl Acad Sci U S A. 1987 Apr;84(7):1824-8. doi: 10.1073/pnas.84.7.1824.

Authors

J Martinez-Izquierdo¹, T Hohn

Affiliation

¹ Friedrich Miescher-Institut, P.O. Box 2543, CH-4002 Basel, Switzerland.

Abstract

A protein kinase has been found to be associated with particles of the plant virus cauliflower mosaic virus. This protein kinase can phosphorylate endogenous viral capsid proteins in vitro and exchange substrates with casein kinase type II. The activity is not affected by cAMP but is enhanced considerably by ADP. The cofactor is either Mn(2+) or Mg(2+), and the phosphate donor is either ATP or GTP. Serine and threonine residues are phosphorylated.