D-six4 plays a key role in patterning cell identities deriving from the Drosophila mesoderm

Dev Biol. 2006 Jun 1;294(1):220-31. doi: 10.1016/j.ydbio.2006.02.044. Epub 2006 Apr 3.

Abstract

Patterning of the Drosophila embryonic mesoderm requires the regulation of cell type-specific factors in response to dorsoventral and anteroposterior axis information. For the dorsoventral axis, the homeodomain gene, tinman, is a key patterning mediator for dorsal mesodermal fates like the heart. However, equivalent mediators for more ventral fates are unknown. We show that D-six4, which encodes a Six family transcription factor, is required for the appropriate development of most cell types deriving from the non-dorsal mesoderm - the fat body, somatic cells of the gonad, and a specific subset of somatic muscles. Misexpression analysis suggests that D-Six4 and its likely cofactor, Eyes absent, are sufficient to impose these fates on other mesodermal cells. At stage 10, the mesodermal expression patterns of D-six4 and tin are complementary, being restricted to the dorsal and non-dorsal regions respectively. Our data suggest that D-six4 is a key mesodermal patterning mediator at this stage that regulates a variety of cell-type-specific factors and hence plays an equivalent role to tin. At stage 9, however, D-six4 and tin are both expressed pan-mesodermally. At this stage, tin function is required for full D-six4 expression. This may explain the known requirement for tin in some non-dorsal cell types.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Body Patterning*
  • Drosophila Proteins / genetics
  • Drosophila Proteins / physiology*
  • Drosophila melanogaster / embryology
  • Embryo, Nonmammalian
  • Eye Proteins / genetics
  • Gene Expression Regulation, Developmental
  • Homeodomain Proteins / physiology*
  • Mesoderm / cytology*
  • Muscles
  • Nerve Tissue Proteins / physiology*
  • Repressor Proteins / genetics
  • Trans-Activators / genetics
  • Transcription Factors / physiology*

Substances

  • Drosophila Proteins
  • Eye Proteins
  • Homeodomain Proteins
  • Nerve Tissue Proteins
  • Repressor Proteins
  • Six4 protein, Drosophila
  • Trans-Activators
  • Transcription Factors
  • eya protein, Drosophila
  • tin protein, Drosophila