Soluble guanylyl cyclase inhibitor prevents Sema3F-induced collapse of axonal and dendritic growth cones of dentate granule cells

Biol Pharm Bull. 2006 Apr;29(4):796-8. doi: 10.1248/bpb.29.796.


Controlling axon and dendrite elongation is critical in developing precise neural circuits. Using isolated cultures of dentate granule neurons, we succeeded in simultaneously monitoring the behaviors of axonal and dendritic outgrowth. Our previous study shows that cAMP contributes differentially to Sema3F-induced responses of axons and dendrites, but we report here that the cGMP modulation does not have such a striking axo-dendritic difference. Treatment with Sema3F induced collapse of about 90% growth cones, and pretreatment with 1 muM LY83583, an inhibitor of soluble guanylyl cyclase, partially alleviated the collapse of both axons and dendrites. Thus, unlike cAMP, cGMP modulates axonal and dendritic extension in a similar manner.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aminoquinolines / pharmacology
  • Animals
  • Axons / drug effects*
  • COS Cells
  • Chlorocebus aethiops
  • Culture Media, Conditioned
  • Cytoplasmic Granules / drug effects
  • Dendrites / drug effects*
  • Dentate Gyrus / cytology*
  • Dentate Gyrus / drug effects
  • Enzyme Inhibitors / pharmacology*
  • Growth Cones / drug effects*
  • Guanylate Cyclase / antagonists & inhibitors*
  • Intracellular Signaling Peptides and Proteins / antagonists & inhibitors
  • Intracellular Signaling Peptides and Proteins / physiology*
  • Nerve Tissue Proteins / antagonists & inhibitors
  • Nerve Tissue Proteins / physiology*
  • Rats
  • Rats, Wistar


  • Aminoquinolines
  • Culture Media, Conditioned
  • Enzyme Inhibitors
  • Intracellular Signaling Peptides and Proteins
  • Nerve Tissue Proteins
  • Sema3F protein, rat
  • 6-anilino-5,8-quinolinedione
  • Guanylate Cyclase