A 24-hour screening protocol for identification of vancomycin-resistant Enterococcus faecium

J Clin Microbiol. 2006 Apr;44(4):1578-80. doi: 10.1128/JCM.44.4.1578-1580.2006.


We describe a 24-h protocol for the identification of patients who are positive for vancomycin-resistant Enterococcus faecium (VRE), using stool and rectal swab samples and VRE screening broth, automated DNA extraction, and real-time PCR for vanA and vanB genes. Compared to conventional screening methods, this protocol had a high sensitivity and specificity and a negative predictive value.

MeSH terms

  • Automation
  • Bacterial Proteins / genetics*
  • Carbon-Oxygen Ligases / genetics*
  • Enterococcus faecium / drug effects
  • Enterococcus faecium / genetics
  • Enterococcus faecium / isolation & purification*
  • Feces / microbiology
  • Gram-Positive Bacterial Infections / diagnosis
  • Gram-Positive Bacterial Infections / microbiology*
  • Humans
  • Polymerase Chain Reaction / methods
  • Sensitivity and Specificity
  • Vancomycin / pharmacology
  • Vancomycin Resistance / genetics*


  • Bacterial Proteins
  • VanA ligase, Bacteria
  • VanB protein, Enterococcus
  • Vancomycin
  • Carbon-Oxygen Ligases