The WSTF-SNF2h chromatin remodeling complex interacts with several nuclear proteins in transcription

J Biol Chem. 2006 Jun 16;281(24):16264-71. doi: 10.1074/jbc.M600233200. Epub 2006 Apr 9.

Abstract

The WSTF (Williams syndrome transcription factor) protein is involved in vitamin D-mediated transcription and replication as a component of two distinct ATP-dependent chromatin remodeling complexes, WINAC and WICH, respectively. We show here that the WICH complex (WSTF-SNF2h) interacts with several nuclear proteins as follows: Sf3b155/SAP155, RNA helicase II/Gualpha, Myb-binding protein 1a, CSB, the proto-oncogene Dek, and nuclear myosin 1 in a large 3-MDa assembly, B-WICH, during active transcription. B-WICH also contains RNAs, 45 S rRNA, 5 S rRNA, 7SL RNA, and traces of the U2 small nuclear RNA. The core proteins, WSTF, SNF2h, and nuclear myosin 1, are associated with the RNA polymerase III genes 5 S rRNA genes and 7SL, and post-transcriptional silencing of WSTF reduces the levels of these transcripts. Our results show that a WSTF-SNF2h assembly is involved in RNA polymerase III transcription, and we suggest that WSTF-SNF2h-NM1 forms a platform in transcription while providing chromatin remodeling.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenosine Triphosphatases / chemistry*
  • Cell Nucleus / metabolism
  • Chromatin / chemistry*
  • Chromatin / metabolism
  • Chromosomal Proteins, Non-Histone / chemistry*
  • HeLa Cells
  • Humans
  • Immunoprecipitation
  • Models, Biological
  • Myosins / chemistry
  • Protein Binding
  • RNA / chemistry
  • RNA Polymerase III / metabolism
  • Subcellular Fractions
  • Transcription Factors / chemistry*
  • Transcription, Genetic

Substances

  • BAZ1B protein, human
  • Chromatin
  • Chromosomal Proteins, Non-Histone
  • Transcription Factors
  • RNA
  • RNA Polymerase III
  • Adenosine Triphosphatases
  • SMARCA5 protein, human
  • Myosins