Generation of a mouse with conditionally activated signaling through the BMP receptor, ALK2

Genesis. 2006 Apr;44(4):159-67. doi: 10.1002/dvg.20201.


BMP signaling plays pleiotropic roles in various tissues. Transgenic mouse lines that overexpress BMP signaling in a tissue-specific manner would be beneficial; however, production of each tissue-specific transgenic mouse line is labor-intensive. Here, using a Cre-loxP system, we generated a conditionally overexpressing mouse line for BMP signaling through the type I receptor ALK2 (alternatively known as AVCRI, ActRI, or ActRIA). By mating this line with Cre-expression mouse lines, Cre-mediated recombination removes an intervening floxed lacZ expression cassette and thereby permits the expression of a constitutively active form of Alk2 (caAlk2) driven by a ubiquitous promoter, CAG. Tissue specificity of Cre recombination was monitored by a bicistronically expressed EGFP following Alk2 cDNA. Increased BMP signaling was confirmed by ectopic phosphorylation of SMAD1/5/8 in the areas where Cre recombination had occurred. The conditional overexpression system described here provides versatility in investigating gene functions in a tissue-specific manner without having to generate independent tissue-specific transgenic lines.

Publication types

  • Research Support, N.I.H., Intramural

MeSH terms

  • Activin Receptors, Type I / genetics
  • Activin Receptors, Type I / metabolism*
  • Adenoviridae / genetics
  • Animals
  • Bone Morphogenetic Protein Receptors / metabolism*
  • Crosses, Genetic
  • Female
  • Gene Expression Regulation, Developmental*
  • Green Fluorescent Proteins / metabolism
  • Integrases / genetics
  • Integrases / metabolism
  • Male
  • Mice
  • Mice, Transgenic
  • Models, Genetic
  • Promoter Regions, Genetic
  • Recombination, Genetic
  • Signal Transduction*
  • Transgenes


  • Green Fluorescent Proteins
  • Activin Receptors, Type I
  • Acvr1 protein, mouse
  • Bone Morphogenetic Protein Receptors
  • Cre recombinase
  • Integrases