Transgenic mouse for conditional, tissue-specific Cox-2 overexpression

Genesis. 2006 Apr;44(4):177-82. doi: 10.1002/dvg.20199.


We constructed a cyclooxygenase-2 (Cox-2) conditional overexpression transgenic mouse (Cox-2 COE). The transgene contains a CAG promoter driving the Cox-2 and humanized Renilla luciferase (hRL) coding regions, linked by an internal ribosomal entry site. The promoter is followed by a loxP-flanked sequence containing enhanced green fluorescent protein (EGFP), a neomycin selection cassette, and a transcriptional/translational STOP sequence. In the presence of Cre recombinase the loxP-flanked sequence is excised. Cox-2/hRL expression can be monitored repeatedly and noninvasively in vivo by imaging hRL activity. To demonstrate conditional Cox-2 and hRL expression, a nonreplicating adenovirus carrying Cre recombinase (Ad.CMV.Cre) was injected intravenously; hepatic Cox-2 expression and hRL signal were elevated. Cox-2 COE embryonic fibroblasts express both Cox-2 and hRL following Ad.CMV.Cre infection. PGE(2) production is also increased following Ad.CMV.Cre infection of Cox-2 COE embryo fibroblasts. Cox-2 COE mice should be valuable for the study of Cox-2 overexpression in cardiovascular disease, acute and chronic inflammatory responses, neurodegenerative diseases, and cancer.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Adenoviridae / genetics
  • Animals
  • Cyclooxygenase 2 / analysis
  • Cyclooxygenase 2 / genetics
  • Cyclooxygenase 2 / metabolism*
  • Fibroblasts / enzymology
  • Fibroblasts / metabolism
  • Gene Deletion*
  • Gene Expression Regulation*
  • Genetic Techniques*
  • Genetic Vectors / administration & dosage
  • Green Fluorescent Proteins / metabolism
  • Injections, Intravenous
  • Integrases / genetics
  • Integrases / metabolism
  • Liver / enzymology
  • Liver / metabolism*
  • Luciferases, Renilla / metabolism
  • Mice
  • Mice, Transgenic
  • Models, Genetic
  • Promoter Regions, Genetic
  • Transgenes
  • Viral Proteins / genetics
  • Viral Proteins / metabolism


  • Viral Proteins
  • Green Fluorescent Proteins
  • Luciferases, Renilla
  • Cyclooxygenase 2
  • Cre recombinase
  • Integrases